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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -RasGAP, CT (Ras GTPase Activating Protein, GAP)

Anti -RasGAP, CT (Ras GTPase Activating Protein, GAP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
Catalog #R1198-13C
Application(s)
Immunoprecipitation (1:1'000; only CT RasGAP; see technical note)
Western Blot (1:1'000-1:3'000)
RasGAP (Ras-specific GTPase-activating protein) is one of the few signalling proteins cleaved by caspases during apoptosis [1-3]. The kinetics of RasGAP cleavage is identical to well-known caspase substrates such as PARP or the caspase themselves. RasGAP cleavage by caspases generates a carboxy-terminal fragment of about 64kD (fragment C) containing the GAP activity and a N-terminal fragment (fragment N) of about 56kD. The latter fragment can be further cleaved into two other smaller fragments, one of which contains the SH2 and SH3 domains. The fact that RasGAP is an abundant ubiquitous protein readily cleaved during apoptosis makes it a very good apoptotic marker. RasGAP is a unique caspase substrate because it can either inhibit or promote apoptosis depending on the extent of its cleavage by caspases. At low levels of caspase activity, generation of fragment N induces a potent Ras-PI3K-Akt-dependent survival pathway. At higher levels of caspase activity, fragment N is further cleaved into two fragments (N1 and N2) that potentiate apoptosis. RasGAP has recently been qualified as an “apoptostat” because it functions as a sensor of caspase activity to determine whether or not a cell should survive [2].
Clone TypePolyclonal
HostRabbit
SourceHuman
FormLiquid. 100µl serum containing 0.02% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to aa 1034-1047 of human RasGAP (Ras-specific GTPase-activating protein).
SpecificityRecognizes human, mouse, rat and monkey full-length RasGAP as well as the C-terminal (CT) fragment generated by caspase cleavage (RasGAP aa 456-1047). Detects a band of ~150kD by Western blot. Does not cross-react with the N-terminal portion of RasGAP (including the SH2 and SH3 domains).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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