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You are here:Home » Antibodies » Abs to Retinoblastoma Proteins » Anti -RBL2 (Retinoblastoma-like Protein 2, Retinoblastoma-related Protein 2, RBR-2, PRB2, 130kD Retinoblastoma-associated Protein, p130, RB2)

Anti -RBL2 (Retinoblastoma-like Protein 2, Retinoblastoma-related Protein 2, RBR-2, PRB2,
130kD Retinoblastoma-associated Protein, p130, RB2)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Two retinoblastoma-related proteins, p107 and pRb2/p130 are structurally and functionally similar to the product of the retinoblastoma gene (pRb/p105). Like pRb they bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Both proteins play a fundamental role in growth control. The highest percentage of undetectable levels, tightest inverse correlation with histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2/p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
Catalog #R1200-50K
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2hrs at RT
Immunoprecipitation: 2ug/mg protein lysate
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlTonsil, A427 cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoSPM301
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to the aa878-913 of p130.
SpecificityRecognizes p130. Species Crossreactivity: human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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