| Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. |
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| Catalog # | R1310-01 |
| Applications | Suitable for use in Western Blot. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 1:10,000–1:30,000 when detected with an anti-chicken horseradish peroxidase-conjugated secondary by ECL or by a colorimetric assay using DAB. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906: IgY, Chicken Pab Rb x Ch |
| I1906-10: IgY, Chicken (HRP) Rb x Ch |
| I1906-12: IgY, Chicken (FITC) Rb x Ch |
| I1906-14: IgY, Chicken (AP) Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 10mM Tris, pH 7.5, 100mM sodium chloride, 0.02% sodium azide. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Recombinant red fluorescent protein (RFP), highly purified by affinity chromatography on Nickel column. |
| Specificity | Recognizes red fluorescent protein (RFP). Shows no reactivity to GFP. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
