Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Red Fluorescent Protein (RFP)

Anti -Red Fluorescent Protein (RFP)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified B
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.
Catalog #R1310-01
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:10,0001:30,000 when detected with an anti-chicken horseradish peroxidase-conjugated secondary by ECL or by a colorimetric assay using DAB.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken (HRP) Rb x Ch
I1906-12: IgY, Chicken (FITC) Rb x Ch
I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgY
HostChicken
Concentration~1mg/ml
FormSupplied as a liquid in 10mM Tris, pH 7.5, 100mM sodium chloride, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenRecombinant red fluorescent protein (RFP), highly purified by affinity chromatography on Nickel column.
SpecificityRecognizes red fluorescent protein (RFP). Shows no reactivity to GFP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links