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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Red Fluorescent Protein (RFP)

Anti -Red Fluorescent Protein (RFP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.
Catalog #R1310-01A
ApplicationsSuitable for use in ELISA, Western Blot, Dot Blot, Immunoprecipitation and Immunostaining. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10K338
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.2, 0.1% sodium azide.
PurityPurified
ImmunogenRed Fluorescent Protein (RFP) from Discosoma and anemone N-terminal peptide, conjugated to KLH.
SpecificityRecognizes native and denatured forms of RFP and its variants tag-RFP, turbo-RFP, DeRed, mCherry and mOrange.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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