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Specifications

The recombinant RFP (dsRed) was expressed and purified from transformed E. coli using a method that ensures high purity and maximal RFP fluorescence. Endotoxin was removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a 27.6kDa monomers containing a N-terminus 6x His-Tag with Ex./Em. = 557/585nm, extinction coefficient (e 280nm = 34380 M-1 Cm-1).
Catalog #	R1310-01B
Endotoxin	<0.1ng/ug
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight	27.6 kDa
Source	E. coli
Purity	~97% (SDS-PAGE, HPLC)
Form	Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to a concentration of 1mg/ml.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	Recombinant RFP Protein, Red Fluoroscent Protein, dsRed