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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Reelin is a 400kD, extracellular matrix glycoprotein secreted by several neurons. It is a serine protease that degrades fibronectin and laminin. Reelin also binds lipoprotein receptor superfamily members APOER2 and VLDLR which transduce signals important for neuronal positioning during brain development and synaptic plasticity in the adult brain. In vivo, Reelin undergoes proteolytic processing at two sites, generating three Reelin fragments. The N-terminal domain binds integrin alpha3/beta1, leading to a disruption of neuronal glial cell interactions and inhibition of neuronal migration. Mouse Reelin shares 94% and 96% amino acid identity with human and rat Reelin, respectively in the region immunized.
Catalog #	R1362
Applications	Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: 5-15ug/ml stains immersion fixed frozen sections of mouse embryo.
	Western Blot: 0.1ug/ml stains recombinant mouse reelin.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Mouse
Concentration	~0.2mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity	Purified by immunoaffinity chromatography
Immunogen	Recombinant protein corresponding to aa27-862 of mouse Reelin expressed in NSO cells (Q60841).
Specificity	Recognizes mouse Reelin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.