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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Reelin

Anti -Reelin

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Reelin is a 400kD, extracellular matrix glycoprotein secreted by several neurons. It is a serine protease that degrades fibronectin and laminin. Reelin also binds lipoprotein receptor superfamily members APOER2 and VLDLR which transduce signals important for neuronal positioning during brain development and synaptic plasticity in the adult brain. In vivo, Reelin undergoes proteolytic processing at two sites, generating three Reelin fragments. The N-terminal domain binds integrin alpha3/beta1, leading to a disruption of neuronal glial cell interactions and inhibition of neuronal migration. Mouse Reelin shares 94% and 96% amino acid identity with human and rat Reelin, respectively in the region immunized.
Catalog #R1362
ApplicationsSuitable for use in Direct ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 5-15ug/ml stains immersion fixed frozen sections of mouse embryo.
Western Blot: 0.1ug/ml stains recombinant mouse reelin.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography
ImmunogenRecombinant protein corresponding to aa27-862 of mouse Reelin expressed in NSO cells (Q60841).
SpecificityRecognizes mouse Reelin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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