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Specifications

Clone Host Grade Applications Monoclonal Mouse E B IP IH

Reelin is a 400kD, extracellular matrix glycoprotein secreted by several neurons. It is a serine protease that degrades fibronectin and laminin. Reelin also binds lipoprotein receptor superfamily members APOER2 and VLDLR which transduce signals important for neuronal positioning during brain development and synaptic plasticity in the adult brain. In vivo, Reelin undergoes proteolytic processing at two sites, generating three Reelin fragments. The central fragment is sufficient for binding and signaling via the lipoprotein receptors. Three isoforms of mouse Reelin that differ in their C-terminal fragments have been described. Within its central regions, mouse reelin shares 95% and 98% amino acid sequence identity with human and rat reelin, respectively
Catalog #	R1362-01
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.
Recommended Dilution	Western Blot: 1:1000; detects a band of ~400-450, 300 and 180-200kD.
	Immunohistochemistry: Paraffin-embedded sections
	Optimal dilutions to be determined by the researcher.
Positive Control	IHC labeling of Cajal-Retzius cells in the embryonic mouse telencephalon.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	9C51
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Recombinant fusion protein, corresponding to aa40-189 of Reelin. Cellular Localization
Specificity	Recognizes Reelin. Expected to react with a wide range of species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.