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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Reelin (RELN, RL, Reeler)

Anti -Reelin (RELN, RL, Reeler)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Reelin is a 400 kDa, secreted glycoprotein that belongs to the reelin family of proteins. It is a serine protease that degrades fibronectin and laminin. Neuronal secretion results in neuron migration and patterning. It also binds Apoer2 that contains exon19, resulting in enhanced memory and learning. Mature mouse reelin is 3435 amino acids (aa) in length. It contains one N-terminal reelin domain (aa 27 - 191), followed by eight EGF- like and 15 BNR interspersing sequences. There is a serine protease site between Phe1280 and Asp1286. There are at least three potential reelin isoforms. Two utilize an alternate start site at Met2581, with one of these also showing an additional two aa deletion (Val3429Ser3430). A third isoform shows a premature truncation after Leu3428. Over aa1221-1661 of the precursor, mouse reelin is 93% and 96% aa identical to human and rat reelin, respectively; over aa1-861 of the mature form, mouse reelin is 94% and 96% identical to human and rat reelin, respectively.
Catalog #R1362-01B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 0.5-1.0ug/ml. Detection limit is ~2ng/welll
Western Blot: 0.1-0.2ug/ml. Detection limit is ~10ng/lane under non-reducing conditions and ~25ng/lane under reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No11D1
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a powder lyophilized from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa27-861 of mouse Reelin expressed in NSO cells (Q60841).
SpecificityRecognizes mouse Reelin. Species Sequence Homology: human, 94%; rat, 96%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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