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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified E B

Reelin is a 400 kDa, secreted glycoprotein that belongs to the reelin family of proteins. It is a serine protease that degrades fibronectin and laminin. Neuronal secretion results in neuron migration and patterning. It also binds Apoer2 that contains exon19, resulting in enhanced memory and learning. Mature mouse reelin is 3435 amino acids (aa) in length. It contains one N-terminal reelin domain (aa 27 - 191), followed by eight EGF- like and 15 BNR interspersing sequences. There is a serine protease site between Phe1280 and Asp1286. There are at least three potential reelin isoforms. Two utilize an alternate start site at Met2581, with one of these also showing an additional two aa deletion (Val3429Ser3430). A third isoform shows a premature truncation after Leu3428. Over aa1221-1661 of the precursor, mouse reelin is 93% and 96% aa identical to human and rat reelin, respectively; over aa1-861 of the mature form, mouse reelin is 94% and 96% identical to human and rat reelin, respectively.
Catalog #	R1362-01B
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 0.5-1.0ug/ml. Detection limit is ~2ng/welll
	Western Blot: 0.1-0.2ug/ml. Detection limit is ~10ng/lane under non-reducing conditions and ~25ng/lane under reducing conditions.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	11D1
Host	Rat
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a powder lyophilized from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant corresponding to aa27-861 of mouse Reelin expressed in NSO cells (Q60841).
Specificity	Recognizes mouse Reelin. Species Sequence Homology: human, 94%; rat, 96%
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.