Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Resistin » Anti -Resistin

Anti -Resistin

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family (Resistin, RELM-alpha and RELM-beta) share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake. It is postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis. Recombinant human Resistin is a 19.5kD disulfide-linked homodimeric protein composed of two identical 92 amino acid chains linked by a single disulfide bond.
Catalog #R1585-21A
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrophobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
Applications  
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionELISA: >0.5ug/ml Detects 0.2-0.4ng/well of hResistin by direct ELISA (using 100ul/well antibody solution).
Western Blot 0.1-0.2ug/ml Detects 1.5-3ng/lane of hResistin under either reducing or non-reducing conditions .
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in 0.5X PBS, pH 7.2. Reconstitute with sterile ddH2O or 40-50% glycerol to a concentration > 0.2mg/ml
PurityPuri ed by af nity chromatography.
ImmunogenHighly pure (>98%) recombinant hResistin (human Resistin).
SpecificityRecognizes human Resistin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links