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You are here:Home » Antibodies » Abs to Resistin » Anti -Resistin

Anti -Resistin

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 aminoacids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Catalog #R1585-21C
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabeticdrugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
ApplicationsSuitable for use in ELISA, Western blotting.
Titer Defined by indirect ELISA is >1:100,000 for antibody concentration
1mg/ml, 25ng of antigen are coated per well, and is then defined at a point of
maximal decrease of the titration curve.
Reconstitution Add 0.1ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of
the antibody. In this case clarify the solution by centrifugation.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration1mg/ml
FormSterile filtered and lyophilized from 1mg/ml in 0.05M phosphate buffer, 0.1M NaCl, pH 7.2.
PurityImmunoaffinity chromatography on a column with immobilized human resistin.
ImmunogenRecombinant Human Resistin
SpecificityAmino acid sequence of the recombinant human resistin is 100% homologous to the amino acid sequence of human serum resistin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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