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You are here:Home » Antibodies » Abs to Resistin » Anti -Resistin (ADSF, Adipose Tissue-Specific Secretory Factor, FIZZ3, Found in Inflammatory Zone)

Anti -Resistin (ADSF, Adipose Tissue-Specific Secretory Factor, FIZZ3, Found in
Inflammatory Zone)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Resistance to insulin characterizes type 2 diabetes, the most common form of diabetes. There is a strong link between type 2 diabetes and obesity, as most patients tend to be obese. Thiozolidinediones (TZDs), a new class of anti-diabetic drugs, enhances target-tissue sensitivity to insulin. A screen of genes down regulated by TZD in adipocyte led to the discovery of a new protein hormone called resistin (for resistance to insulin). Resistin, specifically produced and secreted by adipocyte, is present at elevated levels in the blood of obese animals, and is down regulated by fasting and anti-diabetic drugs. Antibody to resistin stimulated glucose uptake and improved insulin sensitivity in obese mice. Some other protein related to resistin has been called resistin-related molecules (RELM-alpha and beta). Resistin family of proteins was also identified as proteins (FIZZ1-3, for Found in Inflammatory zone) involved in allergy and inflammation.
Resistin is also described as serine/cysteine-rich Adipocyte-Specific Secretory Factor (ADSF or FIZZ3). Resistin (pre peptide length: human 108 aa; mouse/rat 114 aa; mol wt ~12.5kD) is characterized by the presence of a hydrophobic signal peptide that is cleaved before its secretion. Resistin proteins show ~55% homology in human and mouse. It is 45-55% related to RELM-alpha/FIZZ1/PMNG1 (Parasite-induced Macrophage Novel Gene 1). RELM-alpha mRNA was most abundant in white adipose tissue. It is also expressed in mammary tissue with significant fat pads. Unlike resistin, RELM-alpha is not expressed in 3T3-L1 adipocyte or in preadipocytes. It is also found in heart, lung and tongue, where resistin is absent. In mice, RELM-alpha/FIZZ1 is found at low levels in a subset of bronchial epithelial cells and in non-neuronal cells adjacent to neurovascular bundles in the peribronchial stroma, and in the wall of the large and small bowel.
Catalog #R1585-22H
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-5ug/ml using Chemiluminescence technique. Resistin is ~9kD.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide R1585-22K
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 14-aa peptide sequence near the N-terminus of mouse resistin
(KLH).
SpecificityRecognizes mouse resistin. No significant sequence was found with RELMs. Species sequence homology: rat 76%, human 35%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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