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Resistin-like Molecule, beta, Recombinant, Human (RELMb)
Resistin-like Molecule, beta, Recombinant, Human (RELMb)
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| RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. The RELM family consists of resistin (also called FIZZ3), RELM-alfa (FIZZ1), RELM-beta (FIZZ2) and RELM-gamma. Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents. RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant. The physiological function of RELM-beta is not known. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice. | | | Catalog # | R1587-20B | | Description | Recombinant Human RELM-beta is a His-Tagged Fusion Protein, 11kD protein containing 90 amino acid residues of the human RELM-beta and 12 additional amino acid residues-HisTag (underlined). | | Amino Acid Sequence | MG STQCSLDS VMDKKIKDVL NSLEYSPSPI SKKLSCASVK SQGRPSSCPA GMAVTGCACG YGCGSWDVQLETTCHCQCSV VDWTTARCCH LTKLRSHHHH HH. | | Specificity | The amino acid sequence of the recombinant human RELM-beta is 100% homologous to the amino acid sequence of the human RELM-beta without signal sequence. | | Applications | Western blotting, ELISA | | Solubility | Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited. | | Stability | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C. | | Molecular Weight | 11kD | | Source | E. coli | | Purity | 95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding. | | Concentration | ~0.5mg/ml | | Form | Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4.0. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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