| RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. The RELM family consists of resistin (also called FIZZ3), RELM-alpha (FIZZ1), RELM-beta (FIZZ2) and RELM-gamma. Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents. RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant. The physiological function of RELM-beta is not known. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice. |
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| Catalog # | R1587-21A |
| Applications | Suitable for use in ELISA, Western blotting. |
| Titer | Defined by indirect ELISA is >1:100,000 for antibody concentration |
| 1mg/ml, 25ng of antigen are coated per well, and is then defined at a point of |
| maximal decrease of the titration curve. |
| Reconstitution | Add 0.1ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of |
| the antibody. In this case clarify the solution by centrifugation. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | 0.1mg/ml |
| Form | Sterile filtered and lyophilized from 1mg/ml in 0.05M phosphate buffer, 0.1M NaCl, pH 7.2. |
| Purity | Immunoaffinity chromatography on a column with immobilized recombinant human RELM-beta. |
| Immunogen | Recombinant human RELM-beta. |
| Specificity | C-terminal His-Tagged fusion protein produced in E. coli, it is a 11kD protein containing 90 amino acid residues of the human RELM beta and 12 additional amino acid residues – His-Tag (underlined). The amino acid sequence of the recombinant human RELM beta is 100% homologous to the amino acid sequence of the human RELM beta without signal sequence. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
