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Resistin-like Molecule, gamma, Recombinant, Mouse (RELMg)
Resistin-like Molecule, gamma, Recombinant, Mouse (RELMg)
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| RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats. Microarray and real-time RT-PCR experiments revealed a repression of RELMgamma mRNA in nasal respiratory epithelium of cigarette smoke-exposed versus untreated rats. The analysis of the physiological tissue-specific expression revealed highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. Promyelocytic HL60 cells transfected with a RELM-gamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELM-gamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation. | | | Catalog # | R1587-55 | | Description | The Recombinant Mouse RELM-gamma is a His -Tagged Fusion Protein having a molecular weight of 11kD containing 86 amino acid residues of the Mouse RELM-gamma and 16 additional amino acid residues – HisTag (bold). MRGSHHHHHH GMASHMTLES IVEKKVKELL ANRDDCPSTV TKTFSCTSIT ASGRLASCPS GMTVTGCACG YGCGSWDIRD GNTCHCQCST MDWATARCCQ LA | | Specificity | The amino acid sequence of the recombinant Mouse RELM-gamma is 100% homologous to the amino acid sequence of the Mouse RELM-g. | | Applications | Western blotting, ELISA | | Solubility | Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited. | | Stability | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C. | | Molecular Weight | 11kD | | Source | E. coli | | Purity | 95% (SDS-PAGE); Two-step procedure using size exclusion chromatography before and after refolding. | | Concentration | ~0.5mg/ml | | Form | Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4.0. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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