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You are here:Home » Molecular Biology » Growth Factors-Resistin » Resistin-like Molecule, gamma, Recombinant, Mouse, Western Blot Positive Control (RELMg, RELM-gamma)

Resistin-like Molecule, gamma, Recombinant, Mouse, Western Blot Positive Control (RELMg,
RELM-gamma)

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Specifications

Resistance to insulin characterizes type 2 diabetes, the most common form of diabetes. There is a strong link between type 2 diabetes and obesity, as most patients tend to be obese. Thiozolidinediones (TZDs), a new class of anti-diabetic, enhances target-tissue sensitivity to insulin. A screen of genes down regulated by TZD in adipocyte led to the discovery of a new protein hormone called resistin (for resistance to insulin). Some other protein related to resistin has been called resistin- related molecules (RELM-alpha and beta). Resistin family of proteins was also identified as proteins (FIZZ1-3, for Found in Inflammatory zone) involved in allegory and inflammation.
Catalog #R1587-55A
RELM-alpha/FIZZ1/PMNG1 (Parasite-induced Macrophage Novel Gene 1 (prepeptides mouse/rat 111aa; ~77% identity) is 63% related to resistin at the C-terminus (38aa). RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats. Highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. RELM-gamma (mouse 111/117aa; rat 111aa; Signal peptide 1-25aa) is a secreted protein of ~11kD).
SourceRecombinant mouse RELM-gamma (mature 86aa), expressed as His-tag protein.
ApplicationsSuitable for use in Western Blot. Not suitable for ELISA or other applications where native protein is required. Other applications not tested.
Recommended DilutionWestern Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityHighly Purified (~95%)
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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