| Respiratory syncytial virus (RSV) is a 100-350 enveloped virus belonging to the Paramyxoviridae, genus Pneumovirus, that causes minor respiratory infections in adults and bronchitis and bronchopneumonia in children. Its genome consists of single stranded, negative sense RNA that codes for 2 non-structural and 8 structural proteins. |
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| Catalog # | R1595-10A |
| Applications | Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry (frozen): 1:50-1:400 |
| Immunohistochemistry (paraffin): 1:50-1:400. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended. |
| Immunofluorescence: 1:5-1:20. Acetone fixation is recommended. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Positive Control | Immunohistochemistry: Use formalin-fixed, paraffin-embedded RSV infected lung. |
| Immunofluorescence: Use acetone-fixed HeLa cells infected with RSV-A2. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1, IgG2a |
| Clone No | 10F365 |
| Host | Mouse |
| Concentration | Not determined |
| Form | Supplied as a liquid, 0.09% sodium azide. |
| Purity | Supernatant |
| Immunogen | RSV strain A2 |
| Specificity | Recognizes the RSV phosphoprotein, fusion and nuclear protein and reacts with human respiratory syncytial virus subtypes A and B. It does not react with tissue culture isolates of influenza types A and B, parainfluenza types 1, 2, 3 & 4b, adenovirus, HSV types1 & 2, VZV, cytomegalovirus, mumps and measles virus, coxsackie B4 virus, poliovirus types 1, 2 & 3 or Echo virus 19. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
