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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Restrictin (Janusin, Tenascin R)

Anti -Restrictin (Janusin, Tenascin R)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Tenascin R is a 180 kD, 1327 amino acid, extracellular matrix glycoprotein belonging to the Tenascin family of adhesion proteins. Tenascin R is selectively expressed in the central nervous system, affecting neurite outgrowth and synaptic functions. Tenascin R isoform 2 shows a deletion of fibronectin type III repeat #6 (aa # 773-862). The human Tenascin R isoform 1 amino acid sequence is 94% identical to the corresponding canine and mouse sequences.
Catalog #R1630-02
ApplicationsWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Tenascin R. The detection limit for rhTenascin R is approximately 2 ng/lane and 20 ng/lane under non-reducing and reducing conditions, respectively.
Immunohistochemistry: This antibody will detect human Tenascin R in cells and tissues. The working dilution is 2-5ug/ml.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Tenascin R. The detection limit for rhTenascin R is approximately 0.3 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
PurityPurified by human Tenascin R affinity chromatography.
ImmunogenNS0-derved rhTenascin R isoform 1
SpecificitySelected for its ability to recognize human Tenascin R in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 1% crossreactivity with rhTenascin C.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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