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You are here:Home » Antibodies » Abs to Retinoblastoma Proteins » Anti -Retinoblastoma, phosphorylated (Ser807/811) (Rb)

Anti -Retinoblastoma, phosphorylated (Ser807/811) (Rb)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1- phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2–4). Cell cycle–dependent phosphorylation by cdk’s inhibits Rb target binding, thus allowing cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin Ecdk2 phosphorylation (6).Specificity of different cdk/cyclin complexes has been observed in vitro (6–8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
Catalog #R1660-05C
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot 1:1000
Immunoprecipitation: 1:200
Immunohistochemistry (Paraffin): 1:300
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues around Ser807/811 of human Rb.
SpecificityRecognizes endogenous levels of human Rb only when phosphorylated at Ser807/811. Does not recognize Rb phosphorylated at other sites. Species Crossreactivity: Monkey and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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