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You are here:Home » Antibodies » Abs to Retinoblastoma Proteins » Anti -Retinoblastoma, phosphorylated (Thr356) (Rb)

Anti -Retinoblastoma, phosphorylated (Thr356) (Rb)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Target Group: Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F-1, PU.1, ATF-2, UBF, Elf-1 and c-abl. This ability to alter transcription is regulated by phosphorylation catalyzed by the cyclin-dependent protein kinases (cdks). Rb contains at least 16 consensus sequences for cdk phosphorylation, but the significance of all of these sites is unclear. Phosphorylation of threonine 356 is catalyzed by the cdk4 complex Cyclin D-cdk4 and plays a role in mediating growth suppression activity of Rb.
Catalog #R1660-23
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.25-0.75ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat cells in high growth phase.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide derived from a region of human Rb that contains threonine 356 (P06400).
SpecificityRecognizes human Rb when phosphorylated at Thr356. Species sequence homology: mouse (80%) rat (87%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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