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You are here:Home » Antibodies » Abs to Retinoblastoma Proteins » Anti -Retinoblastoma, phosphorylated (Thr821) (Rb)

Anti -Retinoblastoma, phosphorylated (Thr821) (Rb)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F-1, PU.1, ATF-2, UBF, Elf-1 and c-Abl. This ability to alter transcription is regulated by phosphorylation catalyzed by the cyclin-dependent protein kinases (cdks). Rb contains at least 16 consensus sequences for cdk phosphorylation, but the significance of all of these sites is unclear. Rb threonine 821 phosphorylation (catalyzed by cdk2) disrupts interaction with proteins containing the sequence LxCxE. Phosphorylation of this site (in additional to serines 780, 807 and 811) also disrupts binding to E2F.
Catalog #R1660-26G
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:1000
Optimal dilutions should be determined by the researcher.
Positive Control Jurkat cells in high growth phase.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.1% sodium azide, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic phosphopeptide derived from a region of human Rb that contains threonine 821.
SpecificityRecognizes Human Rb when phosphorylated at Thr821. Species sequence homology: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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