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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Retinoic Acid Receptor Responder 1 (RARRES1, Tig1)

Anti -Retinoic Acid Receptor Responder 1 (RARRES1, Tig1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
RARRES1 (Retinoic Acid Receptor Responder 1; also Tig1) is a member of the protease inhibitor I47 family of proteins. It is expressed in epithelium, including keratinocytes, plus prostate and breast epithelium. It is considered to be a tumor-suppressor molecule, presumably acting as an inhibitor of carboxypeptidase A. Mouse RARRES1 is 349 amino acids (aa) in length. Based on human, it is a type II transmembrane (TM) protein with a TM segment at, or near, aa75-95. One potential 350aa splice variant exists that shows an almost complete aa divergence after Pro105.
Catalog #R1691-97
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml; Detection limit is ~0.3ng/well.
Western Blot: 1ug/ml
Immunohistochemistry (Fluorescence): 10ug/ml; Detects RARRES1 in cryosections of mouse liver. Tissue was fixed overnight in 4% PFA + 14% picric acid in PBS at 4°C. Sections were blocked with 10% donkey serum and 0.1% Triton X-100 in PBS, 1% BSA for 45 minutes at RT. After blocking, sections were incubated with R1691-97 for 3 hours at RT, followed by donkey anti-goat IgG (NL557) for 1 hour at RT. Between each step, cells were washed with PBS, 0.1% BSA.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenE. coli-derived recombinant mouse RARRES1 (aa108-349), Accession #XP_001475843. Species Sequence Homology: rat (88%), human (79%).
SpecificityRecognizes mouse RARRES1. Does not recognize recombinant human RARRES1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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