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Anti -Retinoic Acid Receptor Responder 1 (RARRES1, Tig1)
Anti -Retinoic Acid Receptor Responder 1 (RARRES1, Tig1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
|
| RARRES1 (Retinoic Acid Receptor Responder 1; also Tig1) is a member of the protease inhibitor I47 family of proteins. It is expressed in epithelium, including keratinocytes, plus prostate and breast epithelium. It is considered to be a tumor-suppressor molecule, presumably acting as an inhibitor of carboxypeptidase A. Mouse RARRES1 is 349 amino acids (aa) in length. Based on human, it is a type II transmembrane (TM) protein with a TM segment at, or near, aa75-95. One potential 350aa splice variant exists that shows an almost complete aa divergence after Pro105. | | | Catalog # | R1691-97 | | Applications | Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Direct ELISA: 0.5-1ug/ml; Detection limit is ~0.3ng/well. | | Western Blot: 1ug/ml | | Immunohistochemistry (Fluorescence): 10ug/ml; Detects RARRES1 in cryosections of mouse liver. Tissue was fixed overnight in 4% PFA + 14% picric acid in PBS at 4°C. Sections were blocked with 10% donkey serum and 0.1% Triton X-100 in PBS, 1% BSA for 45 minutes at RT. After blocking, sections were incubated with R1691-97 for 3 hours at RT, followed by donkey anti-goat IgG (NL557) for 1 hour at RT. Between each step, cells were washed with PBS, 0.1% BSA. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Mouse | | Concentration | ~0.2mg/ml | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | E. coli-derived recombinant mouse RARRES1 (aa108-349), Accession #XP_001475843. Species Sequence Homology: rat (88%), human (79%). | | Specificity | Recognizes mouse RARRES1. Does not recognize recombinant human RARRES1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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