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You are here:Home » Antibodies » Abs to Enzymes » Anti -Reverse Transcriptase, HIV-1 (Human Immunodeficiency Virus-1 RT)

Anti -Reverse Transcriptase, HIV-1 (Human Immunodeficiency Virus-1 RT)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IC IF
Catalog #R1992-05
ApplicationsSuitable for use in ELISA, Western Blot, Conjugation, Immunohistochemistry, Immunocytochemistry and Immunoadsorbance. Other applications have not been tested.
Recommended DilutionConjugation: Enzymes, radiolabels, fluorchromes and other markers.
Optimal dilutions to be determined by the researcher.
SourceProduced by murine hybridoma cells cultured in protein free medium in hollow fiber systems.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Clone TypeMonoclonal
IsotypeIgG1
Clone No2W1
HostMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 10mM Tris HCl, pH 7.4, 15% sucrose. No preservative added. Reconstitute with 500ul sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa387-412 of Reverse Transcriptase, HIV-1 (Human Immunodeficiency Virus-1 RT).
SpecificityRecognizes aa387-412 of Reverse Transcriptase, HIV-1 (Human Immunodeficiency Virus-1 RT). Does not crossreact to HIV-2. The immunoactivity and specificity are tested in ELISA using viral lysate and recombinant proteins. The specificity is confirmed by Western Blot (pattern p51 and p65).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesE.coli plasmid pRC-RT


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