Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse and human RGM-B. The detection limit for rmRGM-B and rhRGM-B is approximately 1 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect mouse RGM-B in cells and tissues. The working dilution is 2-5ug/ml. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse and human RGM-B. The detection limit for rmRGM-B and rhRGM-B is approximately 0.5 ng/well. Optimal dilutions should be determined by each laboratory for each application.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant protein corresponding to Gly49-Ser414 from mouse RGMb, expressed in NS0 cells.

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

Purified by immunoaffinity chromatography.

Recognizes mouse RGMb. Less than 5% crossreactivity was observed with recombinant mouse RGMa and RGMc.