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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -RGS14 (Regulator of G Protein Signaling 14, Regulator of G Protein Signalling 14, Highly Similar to Rap1/rap2 Interacting Protein, Regulation of G Protein Signaling 14)

Anti -RGS14 (Regulator of G Protein Signaling 14, Regulator of G Protein Signalling 14,
Highly Similar to Rap1/rap2 Interacting Protein, Regulation of G Protein Signaling 14)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
RGS14 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP bound form. It contains 1 GoLoco domain, 2 Ras binding (RBD) domains, and 1 RGS domain.
Catalog #R1996-28C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16,000
Western Blot: 0.5-2ug/ml. Detects a band of approximately 65-70 kD (predicted MW: 65kD)
Optimal dilutions to be determined by the researcher.
Cellular Localization Cell Membrane (probable)
Positive Control Jurkat whole cell lysates.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.3 containing, 0.5% BSA and 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide (IGGSLNSTTDSAL) corresponing to residues 335-346 at the C-terminus of human RGS14, according to NP_006471
SpecificityRecognizes human RGS14.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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