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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -RhoA,B,C (GDP/GTP Binding Protein)

Anti -RhoA,B,C (GDP/GTP Binding Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Catalog #R1998-01
ApplicationsSuitable for use in Immunoblot, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionImmunoblot: 0.5-3ug/ml detects Rho in RIPA lysates from Jurkat and HL-60 cells. HL-60 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with 3ug/ml R1998-01. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunocytochemistry: Reported to detect Rho in cells via immunofluorescence.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4F5
HostMouse
SourceHuman
Concentration~0.6mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human RhoA containing amino acids 1-155.
SpecificityRecognizes human Rho (-A, -B, -C), Mr 22kD. Species Crossreactivity: rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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