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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -RhoA,B,C (GDP/GTP Binding Protein)

Anti -RhoA,B,C (GDP/GTP Binding Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Affinity Purified B
Catalog #R1998-01A
ApplicationsSuitable for use in Immunoblot, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:4000 detects Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. HL-60 cell lysate was resolved by electrophoresis, transferred to nitrocellulose
and probed with anti-Rho (-A, -B, -C), (1:2000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Molecular WeightMr 22kD
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No5C94
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility.
SpecificityRecognizes human Rho (-A, -B, -C), Mr 22kD. Recognizes recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins. Species Crossreactivity: Rat. Broad
species cross-reactivity expected based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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