Anti -RhoA,B,C (GDP/GTP Binding Protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Affinity Purified |
B |
|
| Catalog # | R1998-01A | | Applications | Suitable for use in Immunoblot, Immunocytochemistry and Immunofluorescence. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:4000 detects Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. HL-60 cell lysate was resolved by electrophoresis, transferred to nitrocellulose | | and probed with anti-Rho (-A, -B, -C), (1:2000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | Molecular Weight | Mr 22kD | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 5C94 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | KLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility. | | Specificity | Recognizes human Rho (-A, -B, -C), Mr 22kD. Recognizes recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins. Species Crossreactivity: Rat. Broad | | species cross-reactivity expected based on sequence homology. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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