Stable heterohybridoma cell line was obtained by fusion of lymphoblastoid cell line with stable anti-D production with murine myeloma X63-Ag8–653
Applications
Suitable for use in Immunocytochemistry and Blood Group typing. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Anti-D secreting lymphoblastoid cell lines obtained by Epstein-Barr virus transformation of peripheral blood lymphocytes from hyperimmune anti-D donors.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Rho(D) and Rho(Du) human antigens of the Rhesus system. No crossreactivity with other Rhesus antigens.