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R1998-28F Rabbit Anti-ARHGEF11 (Rho Guanine Nucleotide Exchange Factor 11, PDZ-RhoGEF, KIAA0380)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Bo Hu Mk Mo
Accession #
NP_937879
Shipping Temp
Blue Ice
Storage Temp
-20°C

ARHGEF11 is a 1527aa protein with tandem dbl homology and pleckstrin homology domains, PDZ domain, two proline rich sequences and a regulatory G protein-signaling (RGS) sequence. It acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase, but not for RAC1 or CDC42 and acts as GTPase-activating protein (GAP) for GNA12 and GNA13. It forms a complex with G proteins and stimulates neurite through retraction Rho-dependent signals. ARHGEF11 increases glutamate transport 4-fold. It interacts with EAAT4 and modulates its uptake activity and perisynaptic distribution at glutamatergic synapses. It is known to induce the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. It is ubiquitously expressed, in brain, lung, liver, kidney and skeletal muscle.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.5ug/ml Immunohistochemistry (paraffin): 10ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Rat brain lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa1-50 of human ARHGEF11.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ARHGEF11. Species Crossreactivity: chimpanzee, bovine, monkey. Species sequence homology: mouse, rat, canine, chicken, equine.
References
1. Jackson, M. et al. Nature. 410:89-93 (2001). 2. Banerjee, J. et al. Mol. Biol. Cell. 15:1760-1775 (2004).
USBio References
No references available
Conjugates
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