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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IH IC

Rho belongs to the Ras super family of low molecular weight GTPases. Fifteen Rho proteins have already been characterized and divided into three sub-families; the first includes Rho (A, B, C), the second, Rnd 1-3 and the third which includes Rac 1-3, RhoG, Cdc42Hs, Rho/TTF. TC10 and Chp (1-2). Rho acts as a molecular switch which turns on of off various intracellular signaling pathways such as ACK, PAKs, MEKKs ROCK (3). Rho is active when bound to GTP and inactive when bound to GDP (4). It is also known to participate in many physiological activities including cell migration, adhesion, cytokinesis, proliferation, differentiation and apoptosis and to a greater extend cell transformation (5).
Catalog #	R1998-35
Applications	Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:2,000
	Immunohistochemistry: 1:50-1:100
	Immunocytochemistry: 1:100-1:250
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E528
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues in the N-term of human Rho.
Specificity	Recognizes human Rho, NT. Species ccrossreactivity: mouse and rat.
Species sequence Homology	RhoA, RhoB and RhoC.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.