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You are here:Home » Cloning » Cloning-Enzymes » Ribonuclease A, Bovine Pancreas (RNase A)

Ribonuclease A, Bovine Pancreas (RNase A)

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Specifications

Ribonuclease A is used to isolate DNA for RNA-free DNA. Ribonuclease A hydrolyzes RNA at C and U residues, cleaving between the 3-Phosphate group of a pyrimidine nucleotide and the 5-hydroxyl group of the adjacent nucleotide, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains (Volkin and Cohn 1953). The composition and structure of ribonuclease A has been extensively investigated. The tertiary structure was reported by Kartha, et al. (1967) and Lin, (1970). Wang, et al. (1975) indicate that flexibility in a three-dimensional structure is essential for optimal catalytic activity. Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA. The effect of denatured DNA is much greater than that of the native nucleic acid (Sekine et al. 1969).
Catalog #R2011
Activity 60u/mg protein (Assayed by Kunitz method)
Unit DefinitionOne unit will cause the hydrolysis of RNA at that rate where the velocity constant k equals unity (Kunitz units) at 25ºC and pH 5.0.
DNase ( 1.5mg/ml): None Detected
Protease None Detected
SolubilitySoluble at 10mg/ml in analytical grade water. Colorless, clear, complete.
NomenclatureE.C.3.1.27.5; Ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; Ribonuclease 1
Storage and StabilityBest stored desiccated at -20°C or below. Stable for 12 months. However, the enzyme is stable for years stored as a refrigerated dry powder or frozen in aqueous solution.
CAS Number9001-99-4
Molecular Weight13.7kD
SourceBovine pancreas
PurityPurified by ion exchange chromotography (~ 95%).
FormSupplied as a white to off-white powder
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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