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You are here:Home » Antibodies » Abs to Enzymes, Ribonuclease » Anti -Ribonuclease A (RNase A, Pancreatic Ribonuclease, Rib 1, Ribonuclease A Family 1, Ribonuclease Pancreatic, Ribonuclease RNase A Family 1, Ribonuclease RNase A Family 1 Pancreatic, RNAseA, RNase 1, RNASE1, RNS1)

Anti -Ribonuclease A (RNase A, Pancreatic Ribonuclease, Rib 1, Ribonuclease A Family 1,
Ribonuclease Pancreatic, Ribonuclease RNase A Family 1, Ribonuclease RNase A Family 1
Pancreatic, RNAseA, RNase 1, RNASE1, RNS1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IP
Ribonuclease A is used to isolate DNA for RNA-free DNA. Ribonuclease A hydrolyzes RNA at C and U residues, cleaving between the 3-Phosphate group of a pyrimidine nucleotide and the 5-hydroxyl group of the adjacent nucleotide, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains (Volkin and Cohn 1953). The composition and structure of ribonuclease A has been extensively investigated. The tertiary structure was reported by Kartha, et al. (1967) and Lin, (1970). Wang, et al. (1975) indicate that flexibility in a three-dimensional structure is essential for optimal catalytic activity. Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA. The effect of denatured DNA is much greater than that of the native nucleic acid (Sekine et al. 1969).
Catalog #R2011-13
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Conjugation. Other applications not tested.
Recommended DilutionELISA: 1:40,000-1:80,000 use 1ug of R2011-13 in a standard sandwich ELISA using I1904-46Q and ABTS as a substrate for 30 min at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~10mg/ml (UV280)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide.
PurityPuri ed by ion exchange chromatography.
ImmunogenRibonuclease A (Bovine pancreas)
SpecificityRecognizes bovine Ribonuclease A. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum as well as purified and partially purified ribonuclease A (bovine pancreas).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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