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You are here:Home » Antibodies » Abs to Enzymes, Ribonuclease » Anti -Ribonuclease A (RNase A, Pancreatic Ribonuclease, Rib 1, Ribonuclease A Family 1, Ribonuclease Pancreatic, Ribonuclease RNase A Family 1, Ribonuclease RNase A Family 1 Pancreatic, RNAseA, RNase 1, RNASE1, RNS1)

Anti -Ribonuclease A (RNase A, Pancreatic Ribonuclease, Rib 1, Ribonuclease A Family 1,
Ribonuclease Pancreatic, Ribonuclease RNase A Family 1, Ribonuclease RNase A Family 1
Pancreatic, RNAseA, RNase 1, RNASE1, RNS1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP
Ribonuclease A is used to isolate DNA for RNA-free DNA. Ribonuclease A hydrolyzes RNA at C and U residues, cleaving between the 3-Phosphate group of a pyrimidine nucleotide and the 5-hydroxyl group of the adjacent nucleotide, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains (Volkin and Cohn 1953). The composition and structure of ribonuclease A has been extensively investigated. The tertiary structure was reported by Kartha, et al. (1967) and Lin, (1970). Wang, et al. (1975) indicate that flexibility in a three-dimensional structure is essential for optimal catalytic activity. Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA. The effect of denatured DNA is much greater than that of the native nucleic acid (Sekine et al. 1969).
Catalog #R2011-14
ApplicationsSuitable for use in Immunoblot (Western or Dot blot), ELISA, and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 1:5000-1:22,000 (1ug of Ribonuclease A)
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
ConcentrationNot determined.
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified by delipidation and de brination.
ImmunogenRibonuclease A (Bovine Pancreas).
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified ribonuclease A (bovine pancreas).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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