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You are here:Home » Molecular Biology » Cloning-Enzymes » Ribonuclease H (RNase H)

Ribonuclease H (RNase H)

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Specifications

Catalog #R2021-01
Storage Buffer100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100 and 50% glycerol.
Unit DefinitionOne unit of Ribonculease H will produce 1 nmol of acid-soluble ribonuleotides from radiolabeled poly(rA): poly (dT) in 20 minutes at 37°C.
Assay Conditions20mM Tris (pH 7.5), 5mM MgCl2, 2mM DTT, 0.006% BSA
Quality Control Endonuclease/Nickase Activity: None detected after a 14-16 hour incubation of 5 units of enzyme with 300ng of supercoiled plasmid DNA, analyzed on an agarose gel.
Exonuclease ActivityNone detected after a 14-16 hour incubation of 5 units of enzyme with 40ng of 32P labeled Sau3A fragments of pUC19, analyzed by PAGE..
RNase ActivityNone detected after a 14-16 hour incubation of 5 units of enzyme with 25ng of 32P labeled RNA, analyzed by PAGE.
Protease ActivityNone detected after a 14-16 hour incubation of 60 units of enzyme with 1ug of protease substrate, analyzed by fluorescence.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Concentration~10u/ul
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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