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You are here:Home » Antibodies » Abs to Enzymes, Ribonuclease » Anti -Ribonuclease 3 (Protein Drosha, Ribonuclease III, RNase III, p241, DROSHA, RN3, RNASE3L, RNASEN)

Anti -Ribonuclease 3 (Protein Drosha, Ribonuclease III, RNase III, p241, DROSHA, RN3,
RNASE3L, RNASEN)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
Catalog #R2021-75B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 0.5-1.5ug/ml, observed in human liver lysates on ~150kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-KQTDKQKLAQRE, from human RNASEN, at the internal region of the protein (NP_081075.2).
SpecificityRecognizes human RNASEN. Species sequence homology: Canine, rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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