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You are here:Home » Molecular Biology » MB-Cloning-Enzymes » Ribonuclease Inhibitor, Placenta

Ribonuclease Inhibitor, Placenta


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Catalog #R2025A
Storage Buffer20mM HEPES-KOH (pH 7.5), 50mM KCl, 5mM DTT and 50% glycerol.
Unit DefinitionOne unit is the amount of enzyme required to inhibit by 50% the activity of 5ng of RNase A at 25°C (This inhibitor activity is determined by its ability to inhibit hydrolysis of cyclic 2', 3'-CMP by RNase A).
Quality ControlNuclease activity was not detected in any of the following cases, as judged from the intact gel electrophoresis:
1. After incubation of 1ug of DNA-Hind III fragments with 300 units of enzyme for 24 hours at 37°C
2. After incubation of 1ug of pBR322 DNA with 300 units of enzyme for 24 hours at 37°C
3. Migrates as a single band of 50 kd in SDS-polyacrylamide gel electrophoresis.
Note Enzyme inhibits in a wide pH range, but most strongly at pH 7-8. Requires DTT of at least 1 mM to be active.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceHuman Placenta
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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