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You are here:Home » Antibodies » Abs to Enzymes, Ribonuclease » Anti -Ribonuclease P Protein Subunit p20 (RNaseP Protein p20, RPP20, 0610037N12Rik, hPOP7, Processing of Precursor 7 Ribonuclease P/MRP Subunit (S. cerevisiae), POP7, Ribonucleases P/MRP Protein Subunit POP7 Homolog, RPP2)

Anti -Ribonuclease P Protein Subunit p20 (RNaseP Protein p20, RPP20, 0610037N12Rik, hPOP7,
Processing of Precursor 7 Ribonuclease P/MRP Subunit (S. cerevisiae), POP7, Ribonucleases
P/MRP Protein Subunit POP7 Homolog, RPP2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle- specific proteins. On average, snoRNPs contain about 6-10 protein subunits.
Catalog #R2027-90
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: Under less stringent washing conditions, a weaker band of ~60kD is also detected.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5BH5
HostMouse
SourceHuman
ConcentrationNot determined.
FormSupplied as a lyophilized powder from 10mM ammonium bicarbonate buffer, 2mg BSA. Reconstitute with sterile dH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant GST-hRPP20 (Accession #AAC24113, expression vector pGEX-2T), expressed in E.coli.
SpecificityRecognizes human Ribonuclease P Protein Subunit p20.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesSmall Nuclear Ribonucleoprotein 70kDa (U1)


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