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You are here:Home » Molecular Biology » Cloning-Enzymes » Ribonuclease T1, Aspergillus oryzae (RNase T1) (300Ku/mg)

Ribonuclease T1, Aspergillus oryzae (RNase T1) (300Ku/mg)

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Specifications

Ribonuclease T1 is an endonuclease which cleaves RNA or deaminated RNA between guanosine and inosine 3-phosphate residues and the 5-hydroxy residues of adjacent nucleotides with the formation of the corresponding intermediate 2, 3-cyclic phosphates.
Catalog #R2031
Quality ControlCertified free of contaminating non-specific endo-nuclease, exo-nuclease and ribonuclease
Activity 300,000 units/mg protein
Protein AssayAs reported
Unit DefinitionOne unit releases the equivalent of one unit A260 of acid-soluble products from yeast RNA in 15 minutes at 37°C at pH 7.5.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number9026-12-4
Molecular Weight11kD
SourceAspergillus oryzae
PurityChromatographically purified (SDS-PAGE)
Concentration~0.5mg/ml
FormSupplied as a liquid in 2.8M ammonium sulfate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.1.27.3; Guanyloribonuclease; Ribonucleate-3?-guanylo-oligonucleotidohydrolase


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