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You are here:Home » Antibodies » Abs to Ribosomal Proteins » Anti -S6 Ribosomal Protein, phosphorylated (Ser235/236)

Anti -S6 Ribosomal Protein, phosphorylated (Ser235/236)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IP IH
To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a 19 amino acid region in the S6 carboxy-terminus (4,5).
Catalog #R2031-29A
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Good for 10 mini blots.
Immunohistochemistry (paraffin): 1:75
Immunohistochemistry (frozen): 1:75
Immunofluorescence/Immunocytochemistry: 1:100. Fixative: 10% neutral buffered formalin
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5i129
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
PurityPurified
ImmunogenSynthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.
SpecificityDetects endogenous levels of human ribosomal protein S6 only when phosphorylated at serines 235 and 236.
Species CrossreactivityMouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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