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You are here:Home » Antibodies » Abs to Ribosomal Proteins » Anti -S6 Ribosomal Protein, phosphorylated (Ser235/236) (Alexa FluorŪ 647)

Anti -S6 Ribosomal Protein, phosphorylated (Ser235/236) (Alexa FluorŪ 647)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit FC IF
To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a small 19 amino acid region in the S6 carboxy terminus (4,5).
Catalog #R2031-29D
ApplicationsSuitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry 1:10
Immunofluorescence: 1:10
Optimal dilutions to be determined by the researcher.
Storage and StabilityStore at 2-8°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. AlexaFluor conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG
Clone No8A32
HostRabbit
SourceHuman
FormSupplied as a liquid in PBS (pH 7.2), less than 0.1% sodium azide, 2 mg/ml BSA.
PurityAs reported
ImmunogenSynthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.
SpecificityDetects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-5. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Unconjugated antibody reacts with human, monkey, mouse and rat Phospho-S6 Ribosomal protein and it is expected that Phospho-S6 Ribosomal Protein (Ser235/236) Rabbit mAb (Alexa Fluor® 647 conjugate) will also recognize Phospho-S6 in these species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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