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You are here:Home » Antibodies » Abs to Ribosomal Proteins » Anti -S6 Ribosomal Protein, phosphorylated (Ser235/236) (Alexa FluorŪ 488)

Anti -S6 Ribosomal Protein, phosphorylated (Ser235/236) (Alexa FluorŪ 488)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit FC IF
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240 and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
Catalog #R2031-29E
ApplicationsSuitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:10
Flow Cytometry: 1:10
Optimal dilutions to be determined by the researcher.
Storage and StabilityStore at 2-8°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. AlexaFluor conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG
Clone No8A31
HostRabbit
SourceHuman
FormSupplied as a liquid in 0.1% sodium azide, 2 mg/ml BSA. Antibody is conjugated to Alexa Fluor® 488 fluorescent dye
PurityAs reported
ImmunogenSynthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser235 and Ser236 of human S6 ribosomal protein.
SpecificityDetects endogenous levels of S6 ribosomal protein only when phosphorylated at Ser235 and Ser236. Unconjugated antibody reacts with human, mouse, rat and monkey Phospho-S6 Ribosomal protein. It is expected that Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize phospho-S6 ribosomal protein in these species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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