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Anti -Ribosomal Protein S6 (S235), phosphorylated
Anti -Ribosomal Protein S6 (S235), phosphorylated
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation. Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylate the S6 ribosomal protein. Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions. This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors. The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, have been identified and are located within a small 19 amino acid region at its carboxyl terminus. | | | Catalog # | R2031-30 | | Applications | Suitable for use in Immunoblot. Other applications not tested. | | Recommended Dilution | Immunoblot: 0.5-2ug/ml. Detects phosphorylated Ribosomal Protein S6 in lysates from serum stimulated rat 3T3 cells. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa230-242 (C-KRRRL[pS]SLRASTS) of human Ribosomal protein S6 with a N-terminal cysteine added for conjugation. | | Specificity | Recognizes phosphorylated Ribosomal Protein S6, Mr ~30kD. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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