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You are here:Home » Antibodies » Abs to Ribosomal Proteins » Anti -Ribosomal Protein S6 (S235), phosphorylated

Anti -Ribosomal Protein S6 (S235), phosphorylated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation. Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylate the S6 ribosomal protein. Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions. This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors. The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, have been identified and are located within a small 19 amino acid region at its carboxyl terminus.
Catalog #R2031-30
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detected phosphorylated Ribosomal Protein S6 in lysates from serum stimulated rat 3T3 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho Ribosomal Protein S6 (2ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa230-242 (C-KRRRL[pS]SLRASTS) of human Ribosomal protein S6 with a N-terminal cysteine added for conjugation. Species Sequence Homology: rat, chicken and Xenopus.
SpecificityRecognizes phosphorylated Ribosomal Protein S6, Mr ~30kD. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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