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You are here:Home » Antibodies » Antibodies-Ribosomal Proteins » Anti -Ribosomal Protein S6, phosphorylated (Ser235)

Anti -Ribosomal Protein S6, phosphorylated (Ser235)


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Clone Host Grade Applications
Monoclonal Rabbit Supernatant B
S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that is known to be phosphorylated by both p70S6 Kinase as well as RSK as well as Ser240/244 which is thought to only be phosphorylated by p70S6K.
Catalog #R2031-30C1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Positive ControlSerum stimulated 3T3 cell lysate.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9H270
ConcentrationNot determined
FormSupplied as liquid, 0.05% sodium azide.
ImmunogenSynthetic peptide containing the sequence ...RRL[pS]SL... in which pS corresponds to phospho-serine at residue 235 of human ribosomal protein S6. Epitope: Ser235
SpecificityRecognizes ribosomal protein S6 when phosphorylated on Ser235. Species Crossreactivity: human, mouse and rat. Broad species cross-reactivity predicted, due to sequence
homology. Species sequence homologyMouse, rat, chicken and Xenopus-100%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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