| To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, have been identified and are located within a small 19 amino acid region in the S6 carboxy terminus (4,5). |
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| Catalog # | R2031-30D |
| Applications | Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. |
| Immunohistochemistry (Paraffin): 1:100. Heating in citrate buffer required. |
| Immunofluorescence: 1:100. Cell smears only. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 4i8 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. |
| Purity | Supernatant |
| Immunogen | Synthetic peptide corresponding to residues 185-205 of human S6 ribosomal protein (KLH coupled). |
| Specificity | Recognizes endogenous levels of total human S6 ribosomal protein independent of phosphorylation. MW |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
