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You are here:Home » Antibodies » Abs to Ribosomal Proteins » Anti -Ribosomal Protein S6, phosphorylated (Ser235, Ser236)

Anti -Ribosomal Protein S6, phosphorylated (Ser235, Ser236)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
40S ribosomal protein S6 (also known as RPS6) is a ~31kD substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. Phosphorylation of RPS6 is rapamycin and wortmannin-sensitive as its activation is mediated by mTOR and PI3K pathways. Ribosomal protein S6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236 235 240 244 247, due to the positions of these amino acid residues on the a-helix. Hyperphosphorylation of ribosomal protein S6 stimulates protein synthesis that mediates
progression through the cell cycle.
Catalog #R2031-31A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa +/- TNF-a or anisomycin
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to human RPS6 that contains serines 235 and 236. Species sequence homology: mouse and rat.
SpecificityRecognizes human RPS6. Species sequence homology: Rat (100%). Does not cross react with RPS6 phosphorylated on serines 244 and 247.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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