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You are here:Home » Antibodies » Abs to Ribosomal Proteins » Anti -Ribosomal Protein S6, phosphorylated (Ser240/244)

Anti -Ribosomal Protein S6, phosphorylated (Ser240/244)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylate the S6 ribosomal protein. Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, have been identified and are located within a small 19 amino acid region at its carboxyl terminus (4,5).
Catalog #R2031-32
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography and immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser240 and Ser244 of human S6 ribosomal protein (KLH).
SpecificityRecognizes endogenous levels of human ribosomal protein S6 only when phosphorylated at Ser240/244 at ~32kD. Does not detect S6 ribosomal protein phosphorylated at other sites.
Species CrossreactivityMouse, rat, monkey and zebrafish. Species Sequence Homology: Chicken and Xenopus - 100%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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