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You are here:Home » Antibodies » Abs to Ribosomal Proteins » Anti -Ribosomal Protein S6, phosphorylated (Ser244, Ser247)

Anti -Ribosomal Protein S6, phosphorylated (Ser244, Ser247)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
40S ribosomal protein S6 (also known as RPS6) is a ~31kD substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. Phosphorylation of RPS6 is rapamycin and wortmannin-sensitive as its activation is mediated by mTOR and PI3K pathways. Ribosomal protein S6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236 235 240 244 247, due to the positions of these amino acid residues on the -helix. Hyperphosphorylation of ribosomal protein S6 stimulates protein synthesis that mediates
progression through the cell cycle.
Catalog #R2031-32B
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000 dilution.
Optimal dilutions to be determined by the researcher.
Recommended Positive Control
HeLa +/- TNF-a or anisomycin.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS pH 7.3,1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic phosphopeptide derived from the region of human RPS6 that contains serines 244 and 247. The sequence is conserved in mouse and rat.
SpecificityRecognizes human RPS6. Species Sequence Homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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