| One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1, 2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2, 3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240 and Ser244) located within a small, carboxy-terminal region of the S6 protein (4, 5). |
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| Catalog # | R2031-32D |
| Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:1000. Incubate membrane with diluted R2031-32D in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. |
| Immunoprecipitation: 1:100 |
| Immunohistochemistry (Paraffin): 1:100 |
| Immunofluorescence (IF-IC): 1:25 |
| Flow Cytometry: 1:25 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 9B147 |
| Host | Mouse |
| Concentration | Not determined |
| Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol. |
| Purity | Serum |
| Immunogen | GST-S6 ribosomal protein (full length) fusion protein. |
| Specificity | Recognizes endogenous levels of total S6 ribosomal protein independent of phosphorylation at ~32kD. Species Crossreactivity: Drosophila, human, mouse, monkey, rat |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
