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You are here:Home » Antibodies » Abs to Lectins » Anti -Ricin, RCA60

Anti -Ricin, RCA60

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E
The ricin toxin consists of two chains, A and B, covalently linked by a disulfide bond. The A chain acts as a glycosidase that removes an adenine residue from the 28S rRNA. The area from which the adenine is removed is essential for binding of elongation factors, destroying it leaves the ribosome inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, much faster than the cell can replace them, meaning that a single ricin A chain can kill a cell. The B chain binds to complex carbohydrates on the cell surface to facilitate entry of the A chain into the cell. Without the B chain, the A chain has very low toxicity. Ricin is easily extracted from the castor bean (Ricinus communis), and the lethal dose for humans is very small. It is toxic when inhaled, ingested or injected and there is no known antidote, although a vaccine has been developed. Treatment is symptomatic and supportive, with long term organ damage likely in survivors. The ease of extraction and distribution makes it a potential candidate for biological warfare although it is less stable than other agents, such as anthrax.
Catalog #R2031-53D
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 0.01M phosphate, pH 7.4, 0.9% sodium chloride. Reconstitute with 1ml sterile ddH2O.
PurityPurified by ammonium sulfate precipitation.
ImmunogenRicin A chain toxoid (RCA60)
SpecificityRecognizes the A chain of Ricin toxin and toxoid. Minimal crossreactivity with staphylococcal enterotoxins A through E, ET, TSST, and alpha-hemolysin and streptococcal pyrogenic exotoxins A, B and C.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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