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You are here:Home » Antibodies » Abs to Signal Transduction » Anti -Rictor (Rapamycin Insensitive Companion of mTOR, Rapamycin-insensitive Companion of mTOR, RICTR, AVO3, DKFZp686B11164, KIAA1999, Likely Ortholog of Mouse TORC2 Specific Protein AVO3 (S. cerevisiae), mAVO3, MGC39830, Pianissimo)

Anti -Rictor (Rapamycin Insensitive Companion of mTOR, Rapamycin-insensitive Companion of
mTOR, RICTR, AVO3, DKFZp686B11164, KIAA1999, Likely Ortholog of Mouse TORC2 Specific
Protein AVO3 (S. cerevisiae), mAVO3, MGC39830, Pianissimo)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GbL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GbL, Sin1 and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).
Catalog #R2031-62H
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No10g89
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to the sequence around Leu1121 of human rictor protein.
SpecificityRecognizes endogenous levels of total rictor protein. Species crossreactivity: Human and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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