Anti -Rictor (Rapamycin Insensitive Companion of mTOR, Rapamycin-insensitive Companion of
mTOR, RICTR, AVO3, DKFZp686B11164, KIAA1999, Likely Ortholog of Mouse TORC2 Specific
Protein AVO3 (S. cerevisiae), mAVO3, MGC39830, Pianissimo)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP |
|
| Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GbL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GbL, Sin1 and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). | | | Catalog # | R2031-62H | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Immunoprecipitation: 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 10g89 | | Host | Rabbit | | Source | Human | | Concentration | Not determined. | | Form | Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to the sequence around Leu1121 of human rictor protein. | | Specificity | Recognizes endogenous levels of total rictor protein. Species crossreactivity: Human and mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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