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You are here:Home » Molecular Biology » Cloning-Nucleic Acids » RNA MS2 Phage (Ribonucleic Acid)

RNA MS2 Phage (Ribonucleic Acid)

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Specifications

Ribonucleic acids are long-chain polymers of nucleotides linked through 3',5'-phosphodiester bonds. Constituent bases are derivatives of purine and pyrimidines, the sugar is D-ribose. The RNA nucleotide sequence, which is genetic information transferred from DNA, acts as a template in sequencing amino acids in protein synthesis.
Catalog #R2033-18
MS2 RNA is a linear molecule of 3,569 nucleotides with a molecular weight of 1.2x10e6. The RNA from bacteriophage MS2 has been extensively studied as natural RNA in vivo and in vitro protein synthesis. Many of the single steps in initiation, elongation and termination of polypeptide synthesis have been worked out using MS2 RNA. The nucleotide sequence of MS2 RNA has been established facilitating structural and functional studies.
MS2 RNA contains genetic information to specify three viral proteins A-protein (structural), coat protein (structural) and replicase. It contains a high degree of secondary structure.
AppearanceColorless, clear, complete
Size3569bp
Concentration~0.5mg/ml
RNA (A260)~20u/ml
1unit (A260) of RNA is ~40ug
Quality Control Protein Free
Free from host RNA and DNA
Not capped
Product Performance Avoid denaturing agents (e.g. formaldehyde).
Use in 2% agarose gel under native conditions only.
Storage and StabilityStable for 6 months at -20°C. Avoid contamination with RNase from bacteria or human skin. Avoid repeated freeze-thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~1,200kD
SourceMS2 Phage
ConcentrationRNA (A260): ~20u/ml
FormSupplied as a liquid in 10mM Tris-HCl, pH 7.5, 1mM EDTA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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